Composite

Part:BBa_K1300000:Design

Designed by: StuyGem   Group: iGEM14_StuyGem_NYC   (2014-06-16)

recA promoter + Key (taRNA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 198


Design Notes

When designing this construct, we wanted the key (trans sequence) to be transcribed only under a certain condition (when UV light was present). Therefore, the construct was designed with a promoter that would be responsive to DNA damage caused by UV light.

Source

Built from existing parts in the registry though BioBrick assembly. 

recA promoter [1] & trans + dterm [2]

References